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KMID : 0378019670100100055
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New Medical Journal
1967 Volume.10 No. 10 p.55 ~ p.61
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Effect of Feeding and Fasting on Gablet Cells of the small Intestine of the rats
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Abstract
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The author exmined the mucous or goblet cells in the small intestine of mouse fed on three unbalanced diets consisting mostly of protein, fat or carbohydrate and in fast state.
On the 30 th day after feeding and after one or two-day fasting specimens were taken from the upper, middle and lower portion of the small intestine.
The specimens were fixed in 10% formol, Orth or in Regaud-Kopsch solution and made into paraffin sections of 5 ,u. Stainings were done by PAS reaction or with toluidine blue.
In the observation, all of the goblet cells in each 30 crypts and villi, boths of which were cut in the axial direction, of the three portions of the small intestine were counted and the averaged number were considered as total relative number of one animal.
The results were as follows:
1. The total relative number of goblet cells in the small intestine of the mouse fed with normal food averaged 81.3. These cells ivere found in general, more often in the lower portion and were diminished craniard. l~ orthermore these cells were found in a larger number in the crypts.
2. The total relative number of the goblet cells in the mouse fed with fat was larger than the normal value, and was largest in the three groups fed on unbalanced diet, smallest in the protein diet group and was in between the carbohydrate diet group. The decreasing grade of the number of cells in the latter two groups was more or less distinct in the lower portion of the small intestine.
3. The goblet cells in the fasted groups increased in number, particularly in the group of two-day fasting compared with the value of the normal group.
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